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Human LIF enzyme-linked immunoassay kit (EH0096)

Price: Regular price $499.00

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Catalog# EH0096

Assay type Sandwich ELISA

Standard curve range 0.69~500 pg/ml

Sensitivity 0.10 pg/ml (50 μl)；0.36 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01420.01450.0143
0.690.02440.02530.02480.0105
2.060.04320.04360.04340.0291
6.170.09400.09110.09260.0782
18.520.24850.24100.24480.2304
55.560.71050.69250.70150.6872
166.671.78401.68401.73401.7197
500.003.37303.40303.38803.3737

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)163.448.510.5177.761.212.2
Standard Deviation9.62.30.65.42.30.6
Coefficient of Variation(%)5.94.86.23.03.84.9

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum301.85-16.871004.94

Sensitivity

0.10 pg/ml (50 μl)；0.36 pg/ml (10 μl)

Standard curve range

0.69~500 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human LIF into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 93% to 116% with an overall mean recovery of 102%.

Specifications

Product Type

ELISA Kit

Product Name

Human LIF enzyme-linked immunoassay kit (EH0096)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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