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Human IL-3R alpha/CD123 enzyme-linked immunoassay kit (EH0134)

Price: Regular price $499.00

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Catalog# EH0134

Assay type Sandwich ELISA

Standard curve range 156.25~10000 pg/ml

Sensitivity 22.75 pg/ml (50 μl)；129.58 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.04910.04750.0483
156.250.09910.09870.09890.0506
312.500.14880.14890.14890.1006
625.000.24710.25170.24940.2011
1250.000.46880.42760.44820.3999
2500.000.81680.83120.82400.7757
5000.001.51401.51801.51601.4677
10000.002.74102.75602.74852.7002

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)219.21152.63499.9194.91053.13264.2
Standard Deviation12.167.3170.512.858.3145.9
Coefficient of Variation(%)5.55.84.96.55.54.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30804.66-2639.861001600.42

Sensitivity

22.75 pg/ml (50 μl)；129.58 pg/ml (10 μl)

Standard curve range

156.25~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-3R alpha/CD123 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 95% with an overall mean recovery of 86%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-3R alpha/CD123 enzyme-linked immunoassay kit (EH0134)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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