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Human IL-35 (linked heterodimer), C-hFc Tag Protein (HA211212)

Price: Regular price $140.00

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Catalog# HA211212

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  • Human

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Product Details

Product Type

Protein

Product Name

Human IL-35 (linked heterodimer), C-hFc Tag Protein (HA211212)

Predicted molecular mass

75.6 kD

Protein construction description

A DNA sequence encoding the human IL-27 beta protein (Q14213) (Arg 21-Lys 229) and human IL-12A protein (P29459) (Arg 23- Ser 219) linked by a gggsgggsgggsgggs linker was expressed with a hFc tag at the C-terminus.

Accession

Q14213/P29459

Protein construction

IL-27B(21-229)-gggsgggsgggsgggs-IL-12A(23-219) | hFc

Source

HEK293

Bio Activity

Testing in progress.

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

Interleukin 35 (IL-35) is a recently discovered anti-inflammatory cytokine from the IL-12 family. Member of IL-12 family - IL-35 is produced by wide range of regulatory lymphocytes and plays a role in immune suppression. IL-35 can block the development of Th1 and Th17 cells by limiting early T cell proliferation.

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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