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Human IFN-α enzyme-linked immunoassay kit (EH0085)

Price: Regular price $499.00

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Catalog# EH0085

Assay type Sandwich ELISA

Standard curve range 0.41~300 pg/ml

Sensitivity 0.03 pg/ml (50 μl) ；0.24 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01870.01810.0184
0.410.04070.04080.04080.0224
1.230.07670.07530.07600.0576
3.700.17960.17580.17770.1593
11.110.48580.48400.48490.4665
33.331.22601.23401.23001.2116
100.002.56102.59502.57802.5596
300.004.26054.26054.26054.2421

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)11.451.2155.612.057.0175
Standard Deviation0.53.89.80.71.84.2
Coefficient of Variation(%)4.47.36.35.33.12.4

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-0.931.30.21

Sensitivity

0.03 pg/ml (50 μl) ；0.24 pg/ml (10 μl)

Standard curve range

0.41~300 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IFN-alpha into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 85% to 128% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Human IFN-α enzyme-linked immunoassay kit (EH0085)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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