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Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit (EH0214)

Price: Regular price $499.00

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Catalog# EH0214

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 0.20 pg/ml (50 μl)；0.76 pg/ml (10 μl)；

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01130.01170.0115
6.860.02820.02960.02890.0174
20.580.05830.05970.05900.0475
61.730.16150.16270.16210.1506
185.190.40680.45280.42980.4183
555.561.05601.13301.09451.0830
1666.672.21502.18702.20102.1895
5000.003.24003.28903.26453.2530

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(None)98.6567.81800.981.2446.11484.8
Standard Deviation5.241.2140.43.213.970.5
Coefficient of Variation(%)5.37.37.83.93.14.7

Sample Values

Sample MatrixSample EvaluatedRange (None)Detectable (%) Mean of Detectable (None)
Serum300.63-9.141004.14

Sensitivity

0.20 pg/ml (50 μl)；0.76 pg/ml (10 μl)；

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of Human CD254/RANKL/TNFSF11 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 102% with an overall mean recovery of 91%.

Specifications

Product Type

ELISA Kit

Product Name

Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit (EH0214)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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