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Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step) (EHY0196)

Price: Regular price $629.00

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Catalog# EHY0196

Assay type Sandwich ELISA

Standard curve range 1.37~1000 ng/ml

Sensitivity 0.32 ng/ml（10 μl）

Reacts with

  • Human

Safety datasheet

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COA DOWNLOAD

PERFORMANCE CHARACTERISTICS

Product detail

ng/ml O.D. Average Corrected
0.000.01140.01100.0112
1.370.02000.02070.02040.0092
4.120.04130.04490.04310.0319
12.350.11680.11280.11480.1036
37.040.33790.31940.32870.3175
111.110.94530.97120.95830.9471
333.332.34202.42802.38502.3738
1000.003.95703.98353.97033.9591

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(ng/ml)22.4108.9363.219.398.4338.4
Standard Deviation1.15.217.40.42.28.7
Coefficient of Variation(%)5.04.74.81.92.22.6

Sample Values

Sample MatrixSample EvaluatedRange (μg/ml)Detectable (%) Mean of Detectable (μg/ml)
Serum30169.32-423.79100306.49

Sensitivity

0.32 ng/ml（10 μl）

Standard curve range

1.37~1000 ng/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Antithrombin III/ATIII into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 83% to 117% with an overall mean recovery of 101%.

Specifications

Product Type

ELISA Kit

Product Name

Human Antithrombin III/AT III enzyme-linked immunoassay kit(one step) (EHY0196)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P01008

PROTEIN NAME

Antithrombin III/AT III

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