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Human Annexin A6/ANXA6, N-Twin Strep, C-His, Flag Tag Protein (HA210671)

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Catalog# HA210671

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  • Human

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Product Details

Product Type

Protein

Product Name

Human Annexin A6/ANXA6, N-Twin Strep, C-His, Flag Tag Protein (HA210671)

Predicted molecular mass

82.5 kD

Protein construction description

A DNA sequence encoding the human A6/ANXA6 protein (P08133) (Met 1-Asp 673) was expressed with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus.

Accession

P08133

Protein construction

Twin Strep | Annexin A6/ANXA6 (1-673) | His | Flag

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. May associate with CD21. May regulate the release of Ca(2+) from intracellular stores.

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20C to -80C It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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