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Human Agrin, C-His Tag Protein (HA211173)

Price: Regular price $379.00

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Catalog# HA211173

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  • Human

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Product Details

Product Type

Protein

Product Name

Human Agrin, C-His Tag Protein (HA211173)

Predicted molecular mass

104.4 kD

Protein construction description

A DNA sequence encoding the human Agrin protein (O00468) (Ala 1103-Pro 2068) was expressed with a His tag at the C-terminus.

Accession

O00468

Protein construction

Agrin (1103-2068) | His

Source

HEK293

Bio Activity

Testing in progress.

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms.

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20C to -80C It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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