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Human Adiponectin enzyme-linked immunoassay kit (EH0099)

Price: Regular price $499.00

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Catalog# EH0099

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 0.15 pg/ml (50 μl)；3.56 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00970.01020.0100
6.860.02770.02830.02800.0181
20.580.06650.05920.06290.0529
61.730.18970.18830.18900.1791
185.190.56150.54510.55330.5434
555.561.41601.41901.41751.4076
1666.672.89402.79302.84352.8336
5000.004.10264.10654.10464.0946

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)103.3485.31632.6111.5520.81724.6
Standard Deviation2.827.881.65.831.7128.9
Coefficient of Variation(%)2.75.75.05.26.17.5

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum302621.9-7072.81004559.1

Sensitivity

0.15 pg/ml (50 μl)；3.56 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Adiponectin into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 126% with an overall mean recovery of 93%.

Specifications

Product Type

ELISA Kit

Product Name

Human Adiponectin enzyme-linked immunoassay kit (EH0099)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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