HSF2 Recombinant Rabbit Monoclonal Antibody [JE48-40] (ET7109-78)
Catalog# ET7109-78
Rabbit Monoclonal to HSF2
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HSF2 on different lysates with Rabbit anti-HSF2 antibody (ET7109-78) at 1/500 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Daudi cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-78) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: Daudi cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-78) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.