HRP Conjugated Human YKL-40 Recombinant Rabbit Monoclonal Antibody - Detector [PSH13-09] (HA725090H)
Catalog# HA725090H
Rabbit Monoclonal to YKL-40
Tested for
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ELISA(Det)
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ELISA
Reacts with
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Human
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Cynomolgus monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to HRP
Sandwich ELISA analysis of Human YKL-40 matched pair antibodies
Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08]
Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725088) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (HA211030) starting from 11,100 pg/ml to 0 pg/ml and detect antibody (HA725089, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture: HA725088, Human YKL-40 Rabbit mAb [PSH13-08]
Detector: HA725089, Human YKL-40 Rabbit mAb [PSH13-09]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725088) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human YKL-40 protein (HA211030) starting from 11,100 pg/ml to 0 pg/ml and detect antibody (HA725089, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.