HRP Conjugated Human Von Willebrand Factor Recombinant Rabbit Monoclonal Antibody - Detector [PSH09-04] (HA723431H)
Catalog# HA723431H
Rabbit Monoclonal to Human Von Willebrand Factor
Tested for
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ELISA(Det)
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to HRP
Sandwich ELISA analysis of human Von Willebrand Factor matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723058) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Von Willebrand Factor protein (HA210992) starting from 1,000 ng/ml to 0 ng/ml and detect antibody (HA723431H, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723058) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Human Von Willebrand Factor protein (HA210992) starting from 1,000 ng/ml to 0 ng/ml and detect antibody (HA723431H, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.