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HRP Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody (HA1146)

Price: Regular price $30.00

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Catalog# HA1146

Goat Polyclonal to Goat anti-Chicken IgY

Tested for

  • WB

  • ELISA

Reacts with

  • Chicken

Conjugated to HRP

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Specifications

Product Type

Goat polyclonal secondary

Product Name

HRP Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody (HA1146)

Immunogen

Recombinant protein.

Host

Goat

Conjugation

Hrp

Clonality

Polyclonal

APPLICATION DILUTION

  • WB

  • 1:10,000-1:50,000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Concentration

2ug/ul

Purification

Immunogen affinity purified.

Isotype

IgG

TARGET

PROTEIN NAME

Goat anti-Chicken IgY

FUNCTION

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

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