Hippo Signaling Antibody Kit (HAK21009)
Catalog# HAK21009
Reacts with
-
Human
Western blot analysis of Phospho-YAP1 (S127) on different lysates with Rabbit anti-Phospho-YAP1 (S127) antibody (ET1611-69) at 1/5,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lysates/proteins at 10 µg/Lane.
Lane 7: C6 whole cell lysate
Lane 8: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 9: C6 treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Predicted band size: 54 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 6 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-69) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3: HeLa treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lane 5: NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Lysates/proteins at 10 µg/Lane.
Lane 7: C6 whole cell lysate
Lane 8: C6 treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 9: C6 treated with 100nM Calyculin A for 30 minutes then treated with λpp for 1 hour whole cell lysate
Predicted band size: 54 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 6 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-69) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.