HIF-1 alpha Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH07-00] (HA751127)
Catalog# HA751127
Rabbit Monoclonal to HIF-1 alpha
Tested for
-
WB
-
IF-Cell
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IHC-P
Reacts with
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Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (HA751127) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HepG2 treated with 100μM CoCl2 for 4 hours cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C2C12 treated with 100μM CoCl2 for 4 hours cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 120 kDa
Exposure time: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751127) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (HA751127) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours cell lysate
Lane 3: HepG2 cell lysate
Lane 4: HepG2 treated with 100μM CoCl2 for 4 hours cell lysate
Lane 5: C2C12 cell lysate
Lane 6: C2C12 treated with 100μM CoCl2 for 4 hours cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 120 kDa
Exposure time: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751127) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.