heavy chain Myosin / MYH3 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH18-12] (HA751658)
Catalog# HA751658
Rabbit Monoclonal to heavy chain Myosin / MYH3
Tested for
-
WB
-
IHC-P
-
IP
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of heavy chain Myosin / MYH3 on different lysates with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/5,000 dilution.
Lane 1: RD cell lysate (20 µg/Lane)
Lane 2: Mouse embryo tissue lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (negative) (20 µg/Lane)
Lane 4: Rat embryo tissue lysate (20 µg/Lane)
Lane 5: Rat testis tissue lysate (negative) (20 µg/Lane)
Predicted band size: 224 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751658) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of heavy chain Myosin / MYH3 on different lysates with Rabbit anti-heavy chain Myosin / MYH3 antibody (HA751658) at 1/5,000 dilution.
Lane 1: RD cell lysate (20 µg/Lane)
Lane 2: Mouse embryo tissue lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (negative) (20 µg/Lane)
Lane 4: Rat embryo tissue lysate (20 µg/Lane)
Lane 5: Rat testis tissue lysate (negative) (20 µg/Lane)
Predicted band size: 224 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751658) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.