GWL Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH07-69] (HA751181)
Catalog# HA751181
Rabbit Monoclonal to GWL
Tested for
-
WB
-
IF-Cell
-
IP
Reacts with
-
Human
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of GWL on different lysates with Rabbit anti-GWL antibody (HA751181) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: LNCaP cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 110 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751181) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: LNCaP cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 110 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751181) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.