GST3 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [11D5-R] (HA610147)
Catalog# HA610147
Mouse Monoclonal to GST3
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of GST3 on different lysates with Mouse anti-GST3 antibody (HA610147) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: HT-29 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: K-562 cell lysate
Lane 7: JAR cell lysate
Lane 8: Human brain tissue lysate
Lane 9: Human lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: HT-29 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: K-562 cell lysate
Lane 7: JAR cell lysate
Lane 8: Human brain tissue lysate
Lane 9: Human lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.