Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody (HA1034)
Catalog# HA1034
Goat Polyclonal to Goat anti-Rat IgG(H+L)
Tested for
-
WB
-
ELISA
Reacts with
-
Rat
Western blot analysis of Rat IgG on different lysates with Goat anti-Rat IgG(H+L) antibody (HA1034) at 1/1,000 dilution.
Lane 1: Mouse IgG
Lane 2: Rabbit IgG
Lane 3: Rat IgG
Lane 4: Human IgG
Lysates/proteins at 50 ng/Lane.
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA1034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Rabbit Anti-Goat IgG - HRP Secondary Antibody (HA1005) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse IgG
Lane 2: Rabbit IgG
Lane 3: Rat IgG
Lane 4: Human IgG
Lysates/proteins at 50 ng/Lane.
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA1034) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Rabbit Anti-Goat IgG - HRP Secondary Antibody (HA1005) at 1/50,000 dilution was used for 1 hour at room temperature.