Human GM-CSF Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Detector) [PS01-38] (HA721289)
Catalog# HA721289
Rabbit Monoclonal to Human GM-CSF
Tested for
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ELISA(Det)
Reacts with
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Human
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Dog
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Cynomolgus monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
The binding activity of GM-CSF (HA721289) with recombinant human GM-CSF protein.
Immobilized recombinant human GM-CSF protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721289) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Immobilized recombinant human GM-CSF protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721289) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.