GLS2 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH19-71] (HA751726)
Catalog# HA751726
Rabbit Monoclonal to GLS2
Tested for
-
WB
-
IF-Cell
-
IHC-P
-
IP
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of GLS2 on different lysates with Rabbit anti-GLS2 antibody (HA751726) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: MCF7 cell lysate (low expression)
Lane 3: NCI-H1299 cell lysate (low expression)
Lane 4: T-47D cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 60 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751726) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GLS2 on different lysates with Rabbit anti-GLS2 antibody (HA751726) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: MCF7 cell lysate (low expression)
Lane 3: NCI-H1299 cell lysate (low expression)
Lane 4: T-47D cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 60 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751726) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.