GFP Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [SY0243] (HA750116)
Catalog# HA750116
Rabbit Monoclonal to GFP
Tested for
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WB
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IF-Cell
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IF-Tissue
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IP
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IHC-P
Reacts with
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Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of GFP on GFP recombinant protein with Rabbit anti-GFP antibody (HA750116) at 1/20,000 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750116) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 50 ng/Lane.
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750116) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.