Gemin 1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-89] (HA751918)
Catalog# HA751918
Rabbit Monoclonal to Gemin 1
Tested for
-
WB
-
IF-Cell
-
FC
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Gemin 1 on different lysates with Rabbit anti-Gemin 1 antibody (HA751918) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: Hep G2 (Human liver cancer cells) cell lysate
Lane 3: 293T (Human embryonic kidney cells) cell lysate
Lane 4: U-87 MG (Human glioblastoma cells) cell lysate
Lane 5: SH-SY5Y (Human neuroblastoma cells) cell lysate
Lane 6: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751918, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 32 kDa
Observed band size: 35-38 kDa
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: Hep G2 (Human liver cancer cells) cell lysate
Lane 3: 293T (Human embryonic kidney cells) cell lysate
Lane 4: U-87 MG (Human glioblastoma cells) cell lysate
Lane 5: SH-SY5Y (Human neuroblastoma cells) cell lysate
Lane 6: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751918, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 32 kDa
Observed band size: 35-38 kDa