Ganglioside GD2 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [PSH24-41] (HA610426)
Catalog# HA610426
Mouse Monoclonal to Ganglioside GD2
Tested for
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IF-Cell
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FC(Intra)
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: IMR-32 (Human neuroblastoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA610426, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Species: Human
Sample: IMR-32 (Human neuroblastoma cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA610426, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue).