FXR1 Recombinant Rabbit Monoclonal Antibody [JE40-57] (HA721499)
Catalog# HA721499
Rabbit Monoclonal to FXR1
Tested for
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WB
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IHC-P
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IF-Cell
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FC
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (HA721499) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: A549 cell lysate
Lane 3: K-562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: HeLa cell lysate
Lane 7: C2C12 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70/75 kDa
Exposure time: 1 minute 55 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721499) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: A549 cell lysate
Lane 3: K-562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: HeLa cell lysate
Lane 7: C2C12 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70/75 kDa
Exposure time: 1 minute 55 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721499) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.