FOXC1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-89] (HA750914)
Catalog# HA750914
Rabbit Monoclonal to FOXC1
Tested for
-
WB
-
IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of FOXC1 on different lysates with Rabbit anti-FOXC1 antibody (HA750914) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 75 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750914) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 75 kDa
Exposure time: 1 minute 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750914) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.