FITC Conjugated S100 alpha 6 Recombinant Rabbit Monoclonal Antibody [JF0976] (HA724357F)
Catalog# HA724357F
Rabbit Monoclonal to S100 alpha 6
Tested for
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IF-Cell
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to FITC
Immunocytochemistry analysis of C6 cells labeling S100 alpha 6 with Rabbit anti-S100 alpha 6 antibody (HA724357F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-S100 alpha 6 antibody (HA724357F, FITC, green) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-S100 alpha 6 antibody (HA724357F, FITC, green) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.