FITC Conjugated Goat anti-rabbit IgG polyclonal Antibody (HA1124)
Catalog# HA1124
Goat Polyclonal to Goat anti-rabbit IgG
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IF-Cell
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IF-Tissue
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FC
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IHC-Fr
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Rabbit
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA721174) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (FITC, HA1124) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Specifications
Product Type
Goat polyclonal secondary
Product Name
FITC Conjugated Goat anti-rabbit IgG polyclonal Antibody (HA1124)
Immunogen
Rabbit igg (h+l).
Specificity
To minimize cross-reactivity, the fitc conjugated goat anti-rabbit igg (h+l) antibodies have been highly cross-adsorbed against human igg and mouse igg.
Host
Goat
Conjugation
Fitc
Clonality
Polyclonal
APPLICATION DILUTION
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IF-Cell
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1:500-1:1,000
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IF-Tissue
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1:500-1:1,000
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FC
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1:1,000
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IHC-Fr
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
Goat anti-rabbit IgG
FUNCTION
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium.
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