Filaggrin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-66] (HA751907)
Catalog# HA751907
Rabbit Monoclonal to Filaggrin
Tested for
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Filaggrin on different lysates with Rabbit anti-Filaggrin antibody (HA751907) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 3: SK-BR-3 (Human breast cancer cells) cell lysate
Lane 4: SK-MEL-28 (Human malignant melanoma cells) cell lysate
Lane 5: NCI-H441 (Human lung adenocarcinoma cells) cell lysate
Lane 6: NCI-H358 (Human lung adenocarcinoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751907, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 435 kDa
Observed band size: 37,77 kDa
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 3: SK-BR-3 (Human breast cancer cells) cell lysate
Lane 4: SK-MEL-28 (Human malignant melanoma cells) cell lysate
Lane 5: NCI-H441 (Human lung adenocarcinoma cells) cell lysate
Lane 6: NCI-H358 (Human lung adenocarcinoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751907, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 435 kDa
Observed band size: 37,77 kDa