Fibronectin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH14-22] (HA751515)
Catalog# HA751515
Rabbit Monoclonal to Fibronectin
Tested for
-
WB
-
IF-Cell
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FC
-
IP
Reacts with
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Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Fibronectin on different lysates with Rabbit anti-Fibronectin antibody (HA751515) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 272 kDa
Observed band size: 272 kDa
Exposure time: 11 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751515) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 272 kDa
Observed band size: 272 kDa
Exposure time: 11 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751515) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.