FCN1 / M-Ficolin Rabbit Polyclonal Antibody (HA500567)
Catalog# HA500567
Rabbit Polyclonal to FCN1 / M-Ficolin
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
-
Mouse
Western blot analysis of FCN1 / M-Ficolin on different lysates with Rabbit anti-FCN1 / M-Ficolin antibody (HA500567) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa cell lysate treated with deglycosylation
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35/70 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HeLa cell lysate treated with deglycosylation
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 35 kDa
Observed band size: 35/70 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.