F-spondin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH02-84] (HA750837)
Catalog# HA750837
Rabbit Monoclonal to F-spondin
Tested for
-
WB
-
IF-Cell
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FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of F-spondin on different lysates with Rabbit anti-F-spondin antibody (HA750837) at 1/1,000 dilution.
Lane 1: SW1990 cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 91 kDa
Observed band size: 91-120 kDa
Exposure time: 1 minutes 17 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750837) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SW1990 cell lysate
Lane 2: PANC-1 cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 91 kDa
Observed band size: 91-120 kDa
Exposure time: 1 minutes 17 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750837) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.