Estriol Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH15-86] (HA751581)
Catalog# HA751581
Rabbit Monoclonal to Estriol
Tested for
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ELISA
Reacts with
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Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Indirect ELISA analysis of Estriol was performed by coating wells of a 96-well plate with 50 µl per well of Estriol-BSA antigen diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of an Estriol antibody starting at a concentration of 4 µg/mL and serially diluting it to a concentration of 3.9 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.