Epitope Tag Antibody Sampler Kit (HAK21016)
Catalog# HAK21016
Reacts with
-
Species independent
Western blot analysis of GST on different lysates with Rabbit anti-GST antibody (ET1611-47) at 1/50,000 dilution.
Lane 1: 293T transfected with GST cell lysate (2.5 µg/Lane)
Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane)
Exposure time: 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-47) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with GST cell lysate (2.5 µg/Lane)
Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate (15 µg/Lane)
Exposure time: 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-47) at 1/50,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.