Endosomal Marker Antibody Sampler Kit (HAK21027)
Catalog# HAK21027
Reacts with
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Human
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Mouse
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Rat
Western blot analysis of Clathrin heavy chain on different lysates with Rabbit anti-Clathrin heavy chain antibody (ET1704-50) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: HUVEC cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 180kDa
Observed band size: 180 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: HUVEC cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 180kDa
Observed band size: 180 kDa
Exposure time: 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.