Endomucin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-71] (HA751949)
Catalog# HA751949
Rabbit Monoclonal to Endomucin
Tested for
-
WB
-
IHC-P
-
IF-Tissue
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Endomucin on different lysates with Rabbit anti-Endomucin antibody (HA751949) at 1/5,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse thyroid tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse heart tissue lysate
Lane 6: Mouse ovary tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 4 seconds; ECL: K1801
Low expression : Mouse brain (PMID: 12000719).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751949, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 28 kDa
Observed band size: 70-100 kDa
Western blot analysis of Endomucin on different lysates with Rabbit anti-Endomucin antibody (HA751949) at 1/5,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse thyroid tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse heart tissue lysate
Lane 6: Mouse ovary tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 4 seconds; ECL: K1801
Low expression : Mouse brain (PMID: 12000719).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751949, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 28 kDa
Observed band size: 70-100 kDa