Endomucin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-00] (HA751922)
Catalog# HA751922
Rabbit Monoclonal to Endomucin
Tested for
-
WB
-
IHC-P
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Endomucin on different lysates with Rabbit anti-Endomucin antibody (HA751922) at 1/100,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse thyroid tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse heart tissue lysate
Lane 6: Mouse ovary tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Low expression : Mouse brain (PMID: 12000719).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751922, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 28 kDa
Observed band size: 70~100 kDa
Western blot analysis of Endomucin on different lysates with Rabbit anti-Endomucin antibody (HA751922) at 1/100,000 dilution.
Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse thyroid tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse heart tissue lysate
Lane 6: Mouse ovary tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Low expression : Mouse brain (PMID: 12000719).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751922, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 28 kDa
Observed band size: 70~100 kDa