E-Cadherin Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A0-G11-2-R] (HA610047)
Catalog# HA610047
Mouse Monoclonal to E-Cadherin
Tested for
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WB
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IHC-P
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (HA610047) at 1/1,000 dilution.
Lane 1: A431 cell lysate (10 µg/Lane)
Lane 2: SW480 cell lysate (10 µg/Lane)
Lane 3: MCF7 cell lysate (10 µg/Lane)
Predicted band size: 98 kDa
Observed band size: 130 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610047) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate (10 µg/Lane)
Lane 2: SW480 cell lysate (10 µg/Lane)
Lane 3: MCF7 cell lysate (10 µg/Lane)
Predicted band size: 98 kDa
Observed band size: 130 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610047) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.