Dynamin 1 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A1B2-R] (HA610197)
Catalog# HA610197
Mouse Monoclonal to Dynamin 1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Dynamin 1 on different lysates with Mouse anti-Dynamin 1 antibody (HA610197) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610197) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610197) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.