DYKDDDDK tag, AlpHcAbs® Mouse antibody Alpaca Monoclonal (HA710084)
Catalog# HA710084
Alpaca Monoclonal to DYKDDDDK tag
Tested for
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WB
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ICC/IF
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ELISA
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IP
Western blot analysis of DYKDDDDK tag on different lysates with Mouse anti-DYKDDDDK tag antibody (HA710084) at 1/10,000 dilution.
Lane 1: HeLa transfected with DYKDDDDK-tagged Histon H3.1 (N-terminal) cell lysate
Lane 2: L-929 transfected with DYKDDDDK-tagged CD5 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710084) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa transfected with DYKDDDDK-tagged Histon H3.1 (N-terminal) cell lysate
Lane 2: L-929 transfected with DYKDDDDK-tagged CD5 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710084) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.