DNMT1 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A3A8-R] (HA610156)
Catalog# HA610156
Mouse Monoclonal to DNMT1
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (HA610156) at 1/1,000 dilution.
Lane 1: HeLa cell lysate, 20 µg/Lane
Lane 2: 293T cell lysate, 20 µg/Lane
Lane 3: HepG2 cell lysate, 20 µg/Lane
Lane 4: Jurkat cell lysate, 20 µg/Lane
Predicted band size: 183 kDa
Observed band size: 183 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610156) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate, 20 µg/Lane
Lane 2: 293T cell lysate, 20 µg/Lane
Lane 3: HepG2 cell lysate, 20 µg/Lane
Lane 4: Jurkat cell lysate, 20 µg/Lane
Predicted band size: 183 kDa
Observed band size: 183 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610156) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.