DNMT1 Recombinant Mouse Monoclonal Antibody [A3A8-R] (HA601281)
Catalog# HA601281
Mouse Monoclonal to DNMT1
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of DNMT1 on different lysates with Mouse anti-DNMT1 antibody (HA601281) at 1/1,000 dilution.
Lane 1: HeLa cell lysate, 20 µg/Lane
Lane 2: 293T cell lysate, 20 µg/Lane
Lane 3: HepG2 cell lysate, 20 µg/Lane
Lane 4: Jurkat cell lysate, 20 µg/Lane
Predicted band size: 183 kDa
Observed band size: 183 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601281) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate, 20 µg/Lane
Lane 2: 293T cell lysate, 20 µg/Lane
Lane 3: HepG2 cell lysate, 20 µg/Lane
Lane 4: Jurkat cell lysate, 20 µg/Lane
Predicted band size: 183 kDa
Observed band size: 183 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601281) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.