Cystatin C Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH18-60] (HA751684)
Catalog# HA751684
Rabbit Monoclonal to Cystatin C
Tested for
-
WB
-
IHC-P
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (HA751684) at 1/20,000 dilution.
Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 2: MOLT-4 cell lysate (negative) (20 µg/Lane)
Lane 3: Huh7 cell lysate (20 µg/Lane)
Lane 4: TT cell lysate (20 µg/Lane)
Predicted band size: 16 kDa
Observed band size: 14 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751684) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (HA751684) at 1/20,000 dilution.
Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 2: MOLT-4 cell lysate (negative) (20 µg/Lane)
Lane 3: Huh7 cell lysate (20 µg/Lane)
Lane 4: TT cell lysate (20 µg/Lane)
Predicted band size: 16 kDa
Observed band size: 14 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751684) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.