Cyclin E2 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [40-89-R] (HA610048)
Catalog# HA610048
Mouse Monoclonal to Cyclin E2
Tested for
-
WB
-
IHC-P
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IF-Cell
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Cyclin E2 on different lysates with Mouse anti-Cyclin E2 antibody (HA610048) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K-562 cell lysate
Lane 4: A549 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 51/47 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610048) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K-562 cell lysate
Lane 4: A549 cell lysate
Lane 5: MCF7 cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 51/47 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610048) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.