CXCR2 Recombinant Rabbit Monoclonal Antibody [PSH21-28] (HA724211)
Catalog# HA724211
Rabbit Monoclonal to CXCR2
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CXCR2 on different lysates with Rabbit anti-CXCR2 antibody (HA724211) at 1/10,000 dilution.
Lane 1: A549 cell lysate (no heat) (10 µg/Lane)
Lane 2: MDA-MB-231 cell lysate (no heat) (10 µg/Lane)
Lane 3: SK-Br-3 cell lysate (10 µg/Lane)
Lane 4: HepG2 cell lysate (10 µg/Lane)
Lane 5: Neuro-2a cell lysate (no heat) (10 µg/Lane)
Lane 6: 4T1 cell lysate (10 µg/Lane)
Lane 7: PC-12 cell lysate (10 µg/Lane)
Lane 8: C6 cell lysate (10 µg/Lane)
Lane 9: Mouse spleen tissue lysate (no heat) (20 µg/Lane)
Lane 10: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 11: Rat spleen tissue lysate (no heat) (20 µg/Lane)
Lane 12: Rat brain tissue lysate (no heat) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 41 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724211) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (no heat) (10 µg/Lane)
Lane 2: MDA-MB-231 cell lysate (no heat) (10 µg/Lane)
Lane 3: SK-Br-3 cell lysate (10 µg/Lane)
Lane 4: HepG2 cell lysate (10 µg/Lane)
Lane 5: Neuro-2a cell lysate (no heat) (10 µg/Lane)
Lane 6: 4T1 cell lysate (10 µg/Lane)
Lane 7: PC-12 cell lysate (10 µg/Lane)
Lane 8: C6 cell lysate (10 µg/Lane)
Lane 9: Mouse spleen tissue lysate (no heat) (20 µg/Lane)
Lane 10: Mouse brain tissue lysate (no heat) (20 µg/Lane)
Lane 11: Rat spleen tissue lysate (no heat) (20 µg/Lane)
Lane 12: Rat brain tissue lysate (no heat) (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 41 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724211) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.