CSPG4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-56] (HA751946)
Catalog# HA751946
Rabbit Monoclonal to CSPG4
Tested for
-
WB
-
IF-Cell
-
FC
-
IP
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (HA751946) at 1/5,000 dilution.
Lane 1: A375 (Human amelanotic melanoma cells) cell lysate
Lane 2: SK-MEL-28 (Human malignant melanoma cells) cell lysate
Lane 3: MCF7 (Human breast cancer cells) cell lysate
Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 5: SK-BR-3 (Human breast cancer cells) cell lysate
Lane 6: NIH/3T3 (Mouse fibroblasts) cell lysate
Lane 7: N9 (Mouse microglial cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 seconds; ECL: K1801
MCF7 is a negative control (PMID: 20852124).
SK-BR-3 is a negative control (PMID: 20852124).
Negative expression of Cspg4 protein in N9 cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751946, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 251 kDa
Observed band size: 330 kDa
Western blot analysis of CSPG4 on different lysates with Rabbit anti-CSPG4 antibody (HA751946) at 1/5,000 dilution.
Lane 1: A375 (Human amelanotic melanoma cells) cell lysate
Lane 2: SK-MEL-28 (Human malignant melanoma cells) cell lysate
Lane 3: MCF7 (Human breast cancer cells) cell lysate
Lane 4: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 5: SK-BR-3 (Human breast cancer cells) cell lysate
Lane 6: NIH/3T3 (Mouse fibroblasts) cell lysate
Lane 7: N9 (Mouse microglial cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 seconds; ECL: K1801
MCF7 is a negative control (PMID: 20852124).
SK-BR-3 is a negative control (PMID: 20852124).
Negative expression of Cspg4 protein in N9 cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751946, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 251 kDa
Observed band size: 330 kDa