Cofilin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH0-38] (HA750616)
Catalog# HA750616
Rabbit Monoclonal to Cofilin
Tested for
-
WB
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (HA750616) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (30 µg/Lane)
Lane 2: HEK-293 cell lysate (30 µg/Lane)
Lane 3: MCF7 cell lysate (30 µg/Lane)
Lane 4: MDA-MB-468 cell lysate (24 µg/Lane)
Lane 5: K-562 cell lysate (16 µg/Lane)
Lane 6: SH-SY5Y cell lysate (22 µg/Lane)
Lane 7: HUVEC cell lysate (30 µg/Lane)
Lane 8: Jurkat cell lysate (30 µg/Lane)
Lane 9: COS-1 cell lysate (30 µg/Lane)
Lane 10: VERO cell lysate (30 µg/Lane)
Lane 11: NIH/3T3 cell lysate (22 µg/Lane)
Lane 12: PC-12 cell lysate (30 µg/Lane)
Lane 13: Neuro-2a cell lysate (19 µg/Lane)
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 22 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750616) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (30 µg/Lane)
Lane 2: HEK-293 cell lysate (30 µg/Lane)
Lane 3: MCF7 cell lysate (30 µg/Lane)
Lane 4: MDA-MB-468 cell lysate (24 µg/Lane)
Lane 5: K-562 cell lysate (16 µg/Lane)
Lane 6: SH-SY5Y cell lysate (22 µg/Lane)
Lane 7: HUVEC cell lysate (30 µg/Lane)
Lane 8: Jurkat cell lysate (30 µg/Lane)
Lane 9: COS-1 cell lysate (30 µg/Lane)
Lane 10: VERO cell lysate (30 µg/Lane)
Lane 11: NIH/3T3 cell lysate (22 µg/Lane)
Lane 12: PC-12 cell lysate (30 µg/Lane)
Lane 13: Neuro-2a cell lysate (19 µg/Lane)
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 22 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750616) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.