Cleaved PARP Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [SU0314] (HA750136)
Catalog# HA750136
Rabbit Monoclonal to Cleaved PARP
Tested for
-
WB
-
IF-Cell
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
☑ Cell treatment (CT)
Western blot analysis of Cleaved PARP on different lysates with Rabbit anti-Cleaved PARP antibody (HA750136) at 1/2,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 3 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 1 minute 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750136) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Cleaved PARP on different lysates with Rabbit anti-Cleaved PARP antibody (HA750136) at 1/2,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 1μM staurosporine for 3 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 1 minute 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750136) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.