Claudin 4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-48] (HA751896)
Catalog# HA751896
Rabbit Monoclonal to Claudin 4
Tested for
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WB
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IF-Cell
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IHC-P
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FC
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IP
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Claudin 4 on different lysates with Rabbit anti-Claudin 4 antibody (HA751896) at 1/50,000 dilution.
Lane 1: HT-29 (Human colorectal adenocarcinoma cells) cell lysate
Lane 2: SK-MEL-28 (Human malignant melanoma cells) cell lysate
Lane 3: Caco-2 (Human colorectal adenocarcinoma cells) cell lysate
Lane 4: MCF7 (Human breast cancer cells) cell lysate
Lane 5: SW480 (Human colorectal zdenocarcinoma cells) cell lysate
Lane 6: 4T1 (Mouse breast cancer cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 6 seconds; ECL: K1801
Negative expression of claudin-4 protein in SK-MEL-28 cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751896, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 22 kDa
Observed band size: 22 kDa
Western blot analysis of Claudin 4 on different lysates with Rabbit anti-Claudin 4 antibody (HA751896) at 1/50,000 dilution.
Lane 1: HT-29 (Human colorectal adenocarcinoma cells) cell lysate
Lane 2: SK-MEL-28 (Human malignant melanoma cells) cell lysate
Lane 3: Caco-2 (Human colorectal adenocarcinoma cells) cell lysate
Lane 4: MCF7 (Human breast cancer cells) cell lysate
Lane 5: SW480 (Human colorectal zdenocarcinoma cells) cell lysate
Lane 6: 4T1 (Mouse breast cancer cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 6 seconds; ECL: K1801
Negative expression of claudin-4 protein in SK-MEL-28 cells is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751896, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 22 kDa
Observed band size: 22 kDa