Claudin 2 Recombinant Rabbit Monoclonal Antibody [PSH20-92] (HA724187)
Catalog# HA724187
Rabbit Monoclonal to Claudin 2
Tested for
-
WB
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Claudin 2 on different lysates with Rabbit anti-Claudin 2 antibody (HA724187) at 1/5,000 dilution.
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: PANC-1 cell lysate (negative) (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 22 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724187) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Claudin 2 on different lysates with Rabbit anti-Claudin 2 antibody (HA724187) at 1/5,000 dilution.
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: PANC-1 cell lysate (negative) (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 22 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724187) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.